Other

Part:BBa_K268017:Design

Designed by: Nora Yucel   Group: iGEM09_UChicago   (2009-10-21)


YHR139C->GFP


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal EcoRI site found at 2464
    Illegal PstI site found at 1228
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal EcoRI site found at 2464
    Illegal PstI site found at 1228
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal EcoRI site found at 2464
    Illegal BglII site found at 1018
    Illegal BamHI site found at 47
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal EcoRI site found at 2464
    Illegal PstI site found at 1228
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal EcoRI site found at 2464
    Illegal PstI site found at 1228
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 707


Design Notes

ATG is included in primer. Chose to replace YHR139C completely rather than truncate or modify it.


Source

Promoter region from gene YGR035C in S. cerevisiae as obtained through yeastgenome.org. Gene deletion and tagging cassette from Longtine, et al (1998).

References